Type

Recombinant

Description

Proteases(alsocalledProteolyticEnzymes,Peptidases,orProteinases)areenzymesthathydrolyzetheamidebondswithinproteinsorpeptides.Mostproteasesactinaspecificmanner,hydrolyzingbondsat,oradjacentto,specificresidues,oraspecificsequenceofresiduescontainedwithinthesubstrateproteinorpeptide.ProteasesplayanimportantroleinmostdiseasesandBIOLOGicalprocesses,includingprenatalandpostnataldevelopment,reproduction,signaltransduction,immuneresponse,variousautoimmuneanddegenerativediseases,andcancer.Theyarealsoanimportantresearchtool,astheyarefrequentlyusedintheanalysisandproductionofproteins.Glu-CcleavesattheCarboxylsideofE(canalsocleaveDundercertainconditions).RecombinantStaphylococcusGlu-Cisa28.8 kDaproteaseconsistingof266 aminoacidresidues.

AminoAcidSequence

LPNNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQNIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPDAA

Source

E.coli

Purity

95%

BiologicalActivity

CleavesattheCarboxylsideofE(canalsocleaveDundercertainconditions).

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C