Type
Recombinant
Description
RANKLandRANKaremembersoftheTNFsuperfamilyofligandsandreceptorsthatplayanimportantroleintheregulationofspecificimmunityandboneturnover.RANK(receptor)wasoriginallyidentifiedasadendriticcell-membraneprotein,which,byinteractingwithRANKL,augmentstheABIlityofdendriticcells.ThesedendriticcellsthenstimulatenaïveT-cellproliferationinamixedlymphocytereaction,promotethesurvivalofRANK+T-cells,andregulateT-cell-dependentimmuneresponse.RANKL,whichisexpressedinavarietyofcells,includingosteoblasts,fibroblasts,activatedT-cellsandbonemarrowstromalcells,isalsocapableofinteractingwithadecoyreceptorcalledOPG.BindingofsolubleOPGtosRANKLinhibitsosteoclastogenesisbyinterruptingthesignalingbetweenstromalcellsandosteoclasticProgenitorcells,therebyleADIngtoexcessaccumulationofboneandcartilage.RecombinantMurinesRANKLisa19.4 kDapolypeptidecomprisingtheTNF-homologousregionofRANKL(174 aminoacidresidues).
AminoAcidSequence
PAMMEGSWLDVAQRGKPEAQPFAHLTINAASIPSGSHKVTLSSWYHDRGWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPTDYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEFHFYSINVGGFFKLRAGEEISIQVSNPSLLDPDQDATYFGAFKVQDID
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsabilitytoinduceNF-κBinRAW264.7 cellsintheabsenceofanycross-linking.TheexpectedED50 forthiseffectis10.0–25.0 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
