Type
Recombinant
Description
Soluble Receptor Activator of NFĸB Ligand (sRANKL) Mouse Recombinant produced in E.coli is single, non-glycosylated, polypeptide chain containing 179 amino acids and having a total molecular mass of 19.9 kDa. CD254 is purified by proprietary chromatographic techniques.
Amino Acid Sequence
PAMMEGSWLDVAQRGKPEAQPFAHLTINAASIPSGSHKVTLSSWYHDRGWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPTDYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEFHFYSINVGGFFKLRAGEEISIQVSNPSLLDPDQDATYFGAFKVQDID
Source
E. coli
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE
Biological Activity
BioVendor’s rMsRANKL Measured by its ability to induce osteoclast formation on murine RAW264.7 cells using a concentration of 5.0–10.0 ng/ml.
Formulation
The protein (1mg/ml) was lyophilized with 10mM NaP, pH 7.5+ 50 NaCl.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized sRANKL although stable at room temperature for 3 weeks, should be stored desiccated below –18°C. Upon reconstitution sRANKL should be stored at 4°C between 2–7 days and for future use below –18°C. Please prevent freeze-thaw cycles.
Physical Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
