Type
Recombinant
Description
RecombinantHumansRANKLproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining176 aminoacidsandhavingamolecularmassof20006 Dalton.TherHusRANKLispurifiedbyproprietarychromatographictechniques.
AminoAcidSequence
ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeMet-Glu-Lys-Ala-Met.
Source
E.coli
Purity
Greaterthan98.0%asdeterminedby:-(a)AnalysisbyRP-HPLC.-(b)Anion-exchangeFPLC.-©Analysisbyreducingandnon-reducingSDS-PAGESilverStained gel.
BIOLOGicalActivity
Biovendor’s rHusRANKLisfullybiologicallyactivewhencomparedtostandard.TheactivityisdeterminedbyitsABIlitytoinduceosteoclastformationinRAW264.7 cellsusingaconc.of5.0–10.0 ng/mlandbydosedependentstimulationofIL-8 productioninhumanPBMCwhichwasfoundtobe<10ng/ml.
Endotoxin
Lessthan0.1 ng/μg(IEU/μg)ofrHusRANKL.
Formulation
Theproteinwaslyophilizedfromaconcentrated(1mg/ml)solutioncontaining10mMTris,pH-7.6 and50mMsodiumchloride.
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Lyophilizedrec.HumansRANKLalthoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionrHusRANKLshouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
PhysicalAppearance
Sterilefilteredwhitelyophilized(freeze-dried)powder.
