Type

Recombinant

Description

Recombinant HumansRANKL produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 20006 Dalton. The rHusRANKL is purified by proprietary chromatographic techniques.

Amino Acid Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Lys-Ala-Met.

Source

E. coli

Purity

Greater than 98.0% as determined by: - (a) Analysis by RP-HPLC. - (b) Anion-exchange FPLC. - © Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Biological Activity

BioVendor’s rHus­RANKL is fully biologically active when compared to standard. The activity is determined by its ability to induce osteoclast formation in RAW264.7 cells using a conc. of 5.0–10.0 ng/ml and by dose dependent stimulation of IL-8 production in human PBMC which was found to be <10ng/ml.

Endotoxin

Less than 0.1 ng/μg (IEU/μg) of rHusRANKL.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM Tris, pH-7.6 and 50mM sodium chloride.

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Lyophilized rec. Human sRANKL although stable at room temperature for 3 weeks, should be stored desiccated below –18°C. Upon reconstitution rHu sRANKL should be stored at 4°C between 2–7 days and for future use below –18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.