Type

Recombinant

Description

OX40L,amemberoftheTNFsuperfamilyofstructurallyrelatedproteins,existsprimarilyasatypeIImembranebound,non-covalentlylinkedhomotrimericprotein.Itisexpressedonantigenpresentingcells(APCs),suchasdendriticcellsandactivatedB-cells,andalsoonvariousothercellssuchasvascularendothelialcells,mastcells,andnaturalkillercells.OX40LsignalsspecificallythroughtheOX40receptor,whichisexpressedpredominantlyonCD4+TcellsbutalsooncertainactivatedCD8+Tcells.OX40/OX40Lfunctionsasacostimulatorysignal,whichisrequiredforaproductiveinteractionbetweenantigenpresentingcellsandtheirtargetT-cells.Itenhancescellproliferationandsurvival,andincreasesexpressionofRANTES,IL-2,IL-3,andIFNγ.OX40/OX40Lsignalingplaysanimportantroleinimmuno-regulatorycommunication,enablingtheimmunesystemtodistinguishbetween“friendvs.foe”duringactivation;amechanismtypicallytermedimmuno-tolerance.RecombinantOX40Lisaglycosylated133 aminoacidproteincorrespondingtotheextracellularTNFhomologousdomainofthefulllengthtransmembraneprotein.Itmigrateswithanapparentmolecularmassof15.5 –25.0 kDaonSDS-PAGE.

AminoAcidSequence

QVSHRYPRIQSIKVQFTEYKKEKGFILTSQKEDEIMKVQNNSVIINCDGFYLISLKGYFSQEVNISLHYQKDEEPLFQLKKVRSVNSLMVASLTYKDKVYLNVTTDNTSLDDFHVNGGELILIHQNPGEFCVL

Source

Hi-5Insectcells

Purity

97%

BIOLOGicalActivity

DeterminedbyitsABIlitytostimulateIL-8 productionbyhumanPBMCusingaconcentrationrangeof30–100ng/ml. Note:ResultsmayvarywithPBMCdonors.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.5 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C