Type

Recombinant

Description

Slit2isamemberoftheSlitfamilythatsignalsthroughtheRoundabout(Robo)receptorasarepellentforaxonguidanceandneuronalmigration,andalsoactsasachemoattractanttovascularendothelialcellsandachemotaxisinhibitorforleukocytes.Slit2isexpressedprimarilyinthefetallung,kidney,andadultspinalcord,andtoalesserextentintheadultadrenalgland,thyroidandtrachea.Slit2isinitiallysynthesizedasa1499 aminoacidprecursor,whichissubsequentlycleavedintoN-terminalandC-terminalfragments,designatedasSlit2-NandSlit2-Crespectively.Theneurodevelopment-relatedactivities,asmeasuredbytheABIlitytorepelolfactorybulbaxonsandtoinducebranchingindorsalrootgangliaaxons,arecontainedonlyintheN-terminalfragment.RecombinantHumanSlit2-Nisa1093 aminoacidglycoproteincorrespondingtotheN-terminalportionofthefulllengthSlit2precursor,andhasacalculated,theoreticalmolecularweightof122.35 kDa.DuetoglycosylationSlit2-Nmigratesatanapparentmolecularweightofapproximately120.0–140.0 kDabySDS-PAGEanalysisunderreducingconditions.

AminoAcidSequence

QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMV

Source

HEK293cells

Purity

98%

BIOLOGicalActivity

DeterminedbyitsabilitytoinhibitMC3T3/E1osteoblastscelldifferentiation.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstitutein20mMTris,pH8.8+150mMNaCltoaconcentrationof0.1–1.0mg/ml.Note:Slowtodissolve.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.

Storage/Expiration

 –20°C