Type
Recombinant
Description
IL-6 mediatesitsBIOLOGicaleffectsthroughthetypeI IL-6 receptorsystemthatconsistsoftwochains,IL-6Rαandgp130.WhiletheIL-6RαchainisthebindingcomponentspecifictoIL-6,thegp130chainonlytransmitssignalsofIL-6 whenboundtoIL-6Rα.Thegp130canalsotransmitsignalsfromLIF,OSM,CNTF,IL-11 andCT-1 inconjunctionwithotherreceptorsubunits.Thelow-affinitybindingsiteforIL-6 iscomposedofIL-6Rαalone.IL-6Rαisexpressedinawiderangeofcells,includingTcells,fibroblastsandmacrophages.SolubleIL-6Rα,whichconsistsofonlytheextracellulardomainoftheIL-6Rαchain,actsasanagoNISTofIL-6 activityatlowconcentrations.RecombinantHumansIL-6Rαisa37.6 kDaproteinconsistingoftheextracellulardomainoftheIL-6Rαchain(339 aminoacidresidues).
AminoAcidSequence
LAPRRCPAQEVARGVLTSLPGDSVTLTCPGVEPEDNATVHWVLRKPAAGSHPSRWAGMGRRLLLRSVQLHDSGNYSCYRAGRPAGTVHLLVDVPPEEPQLSCFRKSPLSNVVCEWGPRSTPSLTTKAVLLVRKFQNSPAEDFQEPCQYSQESQKFSCQLAVPEGDSSFYIVSMCVASSVGSKFSKTQTFQGCGILQPDPPANITVTAVARNPRWLSVTWQDPHSWNSSFYRLRFELRYRAERSKTFTTWMVKDLQHHCVIHDAWSGLRHVVQLRAQEEFGQGEWSEWSPEAMGTPWTESRSPPAENEVSTPMQALTTNKDDDNILFRDSANATSLPVQD
Source
HEK293cells
Purity
98%
BiologicalActivity
TheED50 wasdeterminedbyitsABIlitytointensifytheIL-6 inducedgrowthinhibitionofmurineM1cellsis≤5.0 ng/ml,inthepresenceof20 ng/mlofrhIL-6.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstitutein1×PBS,pH7.2 toaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
