Type
Recombinant
Description
IL-4 cansignalthroughtypeI andtypeIIreceptorcomplexes,whichshareacommonγchain(γc).ThetypeI receptorcontainsinadditiontotheγchainanIL-4Rαsubunit,whereasthetypeIIreceptorcontainstheIL-13Rα.ThesecretedextracellulardomainofIL-4Rα,calledsIL-4Rα,bindsIL-4 andantagonizesitsactivity.ItplaysanimportantroleinregulatingthedifferentiationofnaiveCD4TcellsandclassswitchingtoIgG1andIgE.RecombinantHumansIL-4Rαisa209 aminoacidproteinthatcorrespondstotheentireextracellulardomainofIL-4Rα.
AminoAcidSequence
GNMKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQLVFLLSEAHTCIPENNGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGNLTVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIYNVTYLEPSLRIAASTLKSGISYRARVRAWAQCYNTTWSEWSPSTKWHNSYREPFEQH
Source
HEK293cells
Purity
98%
BIOLOGicalActivity
TheED50 wasdeterminedbyitsABIlitytoinhibittheIL-4 dependentproliferationofhumanTF-1 cellsis≤5.0 ng/ml(inthepresenceof0.5 ng/mlofIL-4),correspondingtoaspecificactivityof≥2×105 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
