Type
Recombinant
Description
TheIL-2 receptorsystemconsistsofthreenon-covalentlylinkedsubunitstermedIL-2Rα,IL-2Rβ,andIL-2Rγ.TheIL-2RαisatypeI transmembraneproteinconsistingofa219 aminoacidextracellulardomain,a19 aminoacidtransmembranedomainanda13 aminoacidintracellulardomain,whichisnotinvolvedinthetransductionofIL-2 signals.ProteolyticprocessingofIL-2RαreleasestheentireextracellulardomainofIL-2Rα,therebygeneratinga219 aminoacidsolubleproteincalledsolubleIL-2Rα(sIL-2Rα).ThehomodimericformbindsIL-2(KD=10mM)andfacilitatesIL-2 signaling.ThesecretedsIL-2Rαisexpressedonleukemiacells,lymphomacells,andnewlyactivatedTandBcells,aswellasonapproximately10%ofNKcells.RecombinantHumansIL-2Rαisa24.8 kDaproteincontaining219 aminoacidresiduesconsistingofonlytheextracellulardomainofIL-2Rα.Duetoglycosylation,IL-2Rαhasanapproximatemolecularweightof31 kDabasedonSDS-PAGEgelandMassSpectrometry.
AminoAcidSequence
ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSCLVTTTDFQIQTEMAATMETSIFTTEYQ
Source
(BTI-Tn-5B1-4)Hi-5Insectcells*
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytoincreasetheproliferationeffectofIL-2 inmurineCTLL-2 cells. Inthepresenceof1 ng/mlofrecombinantIL-2,theexpectedED50 forthiseffectisbetween0.5–1.5 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstitutein1×PBSpH7.2 toaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
