Type

Recombinant

Description

Total341 AA,UniProtQ6FHJ7(Val19-Val346).MW:39.0 kDa(calculated),migratesat~55 kDaonSDSPAGE;twoN-terminallinkers(2 extraAAand3 extraAAhighlighted),N-terminalFlag-tag(8 extraAA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

PGDYKDDDDKPAGVRGAPCEAVRIPMCRHMPWNITRMPNHLHHSTQENAILAIEQYEELVDVNCSAVLRFFLCAMYAPICTLEFLHDPIKPCKSVCQRARDDCEPLMKMYNHSWPESLACDELPVYDRGVCISPEAIVTDLPEDVKWIDITPDMMVQERPLDVDCKRLSPDRCKCKKVKPTLATYLSKNYSYVIHAKIKAVQRSGCNEVTTVVDVKEIFKSSSPIPRTQVPLITNSSCQCPHILPHQDVLIMCYEWRSRMMLLENCLVEKWRDQLSKRSIQWEERLQEQRRTVQDKKKTAGRTSRSNPPKPKGKPPAPKPASPKKNIKTRSAQKRTNPKRV

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis: >95%

SDS-PAGEGel

12%SDS-PAGEseparationofHumansFRP4
1. M.W.Marker –97,66,45,31,21,14 kDa
2.reducedandboiledsample,2.5μg/lane
3.non-reducedandnon-boiledsample,2.5μg/ lane

Endotoxin

<0.1 EU/µg

Formulation

Filtered(0.4 μm)andlyophilizedin0.5 mg/mLin0.05 Mphosphatebuffer,0.075 MNaCl, pH 7.4

Reconstitution

Add200ulofdeionizedwatertoprepareaworkingstocksolutionof0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.