Type
Recombinant
Description
FasandFasLigand(FasL)belongtotheTNFsuperfamily,andaretypeI andtypeIItransmembraneproteins,respectively.BindingofFasLtoFastriggersapoptosisinFas-bearingcells.Themechanismofapoptosisinvolvesrecruitmentofpro-caspase8 throughanadaptormoleculecalledFADD,followedbyprocessingofthepro-enzymeintoactiveforms.Theseactivecaspasesthencleavevariouscellularsubstrates,leADIngtotheeventualcelldeath.sFasRiscapableofinhibitingFasL-inducedapoptosisbyactingasadecoyreceptorthatservesasasinkforFasL.ThefulllengthFas(receptor)isa319 aminoacidtypeI transmembraneprotein,whichcontainsa157 aminoacidextracellulardomain,a17 aminoacidtransmembranedomain,anda145 aminoacidcytoplasmicdomain.RecombinantHumansolubleFas(sFasReceptor)isa157 aminoacidpolypeptide(17.6 kDa)correspondingtotheTNFR-homologouscysteine-richextracellularFasdomain.
AminoAcidSequence
MRLSSKSVNAQVTDINSKGLELRKTVTTVETQNLEGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCRRCRLCDEGHGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKECTLTSNTKCKEEGSRS
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50wasdeterminedbyitsABIlitytoinhibitthecytotoxicityofJurkatcellsisbetween10–15µg/mlinthepresenceof2ng/mlof hFasL.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
