Type
Recombinant
Description
FasLigand(FasL)isamemberoftheTNFsuperfamilythatisexpressedonthecellsurfaceofactivatedTcells.BindingofFasLtoFasReceptortriggersapoptosisinFas-bearingcells.FasLhastheABIlitytokillTcellsandactivatedBcells,whichleadstodown-regulationoftheimmuneresponse.ThemechanismofFas-inducedapoptosisinvolvesrecruitmentofpro-caspase8 throughanadaptormoleculecalledFADD,followedbyprocessingofthepro-enzymeintoactiveforms.Theseactivecaspasesthencleavevariouscellularsubstrates,leADIngtotheeventualcelldeath.BothhumanandmurinesFasLarefullyactiveonhumanandmurinecells.RecombinantHumansolubleFasLigandisa17.9 kDaproteincomprisingtheTNF-homologousregionofFasLandcontainsan8-residueN-terminalHis-Tag.
AminoAcidSequence
HHHHHHHHPSPPPEKKELRKVAHLTGKSNSRSMPLEWEDTYGIVLLSGVKYKKGGLVINETGLYFVYSKVYFRGQSCNNLPLSHKVYMRNSKYPQDLVMMEGKMMSYCTTGQMWARSSYLGAVFNLTSADHLYVNVSELSLVNFEESQTFFGLYKL
Source
ChineseHamsterOvaryCells(CHO)
Purity
95%
BIOLOGicalActivity
Determinedbyit"s abilitytoinducecytotoxicityinJurkatcellsintheabsenceofanycross-linking.TheED50forthiseffectis10.0 ng/ml,correspondingtoaspecificactivityof1×105units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C
