Type

Recombinant

Description

MurineSF20isabonemarrowstroma-derivedgrowthfactor.SF20isexpressedinbonemarrow,spleenstromacells,restingmononuclearcells,restingCD8+andCD19+cells,andactivatedCD8+Tcells,andhasbeenshowntobindtothesurfaceofcellsexpressingthereceptorTSA-1(ThymicsharedAg-1).AmongitsBIOLOGicalactivities,SF20stimulatestheproliferationofFDCP2cells(amousefactor-dependenthemopoieticcellline)andmouselymphoidcells.RecombinantMurineSF20isa15.8 kDaproteincontaining143 aminoacidresidues.

AminoAcidSequence

MVSEPTTVPFDVRPGGVVHSFSQDVGPGNKFTCTFTYASQGGTNEQWQMSLGTSEDSQHFTCTIWRPQGKSYLYFTQFKAELRGAEIEYAMAYSKAAFERESDVPLKSEEFEVTKTAVSHRPGAFKAELSKLVIVAKAARSEL

Source

E.coli

Purity

98%

BiologicalActivity

Datanotavailable.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C