Type

Recombinant

Description

Total79 AA.MW:8.8 kDa(calculated).UniProtKBacc.no.O95968(Val22-Arg90).N-terminalHis-tag(10 extraAA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

MKHHHHHHASVVCQALGSEITGFLLAGKPVFKFQLAKFKAPLEAVAAKMEVKKCVDTMAYEKRVLITKTLGKIAEKCDR

Source

E.coli

Purity

Purityasdeterminedbydensitometricimageanalysis: >95%

SDS-PAGEGel

14%SDS-PAGEseparationofHumanSCGB1D1:
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandboiledsample,2.5μg/lane
3.non-reducedandnon-boiledsample,2.5μg/lane

Endotoxin

<1.0 EU/ug

Formulation

Filtered(0.4 μm)andlyophilizedfrom0.5 mg/mLin20mMTrisbuffer,50 mMNaCl,pH 7.5

Reconstitution

Add200µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.

Applications

Westernblotting,ELISA

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.