Type

Recombinant

Description

HumansDLL-4 comprisestheextracellularsignalingdomainofDLL,amemberofastructurally-relatedfamilyofsingle-passtypeI trans-membraneproteinsthatserveasligandsforNotchreceptors.DLL-4 functionstospecificallyactivatetheNotch-1 andNotch-4 receptors.TheNotchsignalingpathwayregulatesendothelialcelldifferentiation,proliferationandapoptosis,andisessentialforthedevelopment,maintenanceandremodelingofthevascularsystem.TargeteddeletionoftheDLL-4 geneinmiceresultedinseverevasculardefectsanddeathbeforebirth.Up-regulationofDLL-4 expressionhasbeenimplicatedinthevasculardevelopmentofcertaintumors.ThehumanDLL-4 geneconsistsofa503 aminoacidextracellulardomainwithoneDSLdomain,eightEGF-likerepeats,a21 a.a.transmembranedomain,anda135 a.a.cytoplasmicdomain.RecombinantHumansDLL-4 isa54.3 kDaglycoproteincontaining498 aminoacidresidues.

AminoAcidSequence

SGVFQLQLQEFINERGVLASGRPCEPGCRTFFRVCLKHFQAVVSPGPCTFGTVSTPVLGTNSFAVRDDSSGGGRNPLQLPFNFTWPGTFSLIIEAWHAPGDDLRPEALPPDALISKIAIQGSLAVGQNWLLDEQTSTLTRLRYSYRVICSDNYYGDNCSRLCKKRNDHFGHYVCQPDGNLSCLPGWTGEYCQQPICLSGCHEQNGYCSKPAECLCRPGWQGRLCNECIPHNGCRHGTCSTPWQCTCDEGWGGLFCDQDLNYCTHHSPCKNGATCSNSGQRSYTCTCRPGYTGVDCELELSECDSNPCRNGGSCKDQEDGYHCLCPPGYYGLHCEHSTLSCADSPCFNGGSCRERNQGANYACECPPNFTGSNCEKKVDRCTSNPCANGGQCLNRGPSRMCRCRPGFTGTYCELHVSDCARNPCAHGGTCHDLENGLMCTCPAGFSGRRCEVRTSIDACASSPCFNRATCYTDLSTDTFVCNCPYGFVGSRCEFPVGLP

Source

HEK293cells

Purity

95%

BIOLOGicalActivity

ThesDLL-4,whenimmobilizedatconcentrations>1.5 µg/mLwillinhibitmyogenesisinC2C12 cells.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C