Type

Recombinant

Description

SDF-1αandβarestromal-derived,CXCchemokinesthatsignalthroughtheCXCR4receptor.SDF-1αandβchemoattractBandTcells,andhavebeenshowntoinducemigrationofCD34+stemcells.Additionally,theSDF-1 proteinsexertHIV-suppressiveactivityincellsexpressingtheCXCR4receptor.HumanandmurineSDF-1 proteinsactacrossspecies.SDF-1αandβcontainthefourhighlyconservedcysteineresiduespresentinCXCchemokines.ThematureSDF-1βprotein,producedbyanN-terminaltruncationoftwoadditionalaminoacids,afterremovalofthesignalsequence,contains72 aminoacidresidues.RecombinantRatSDF-1βisan8.4 kDaproteincontaining72 aminoacidresidues.

AminoAcidSequence

KPVSLSYRCPCRFFESHVARANVKHLKILNTPNCALQIVARLKSNNRQVCIDPKLKWIQEYLDKALNKRLKM

Source

E.coli

Purity

95%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanmonocytesusingaconcentrationrangeof100.0–200.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C