Type
Recombinant
Description
SCFisahematopoieticgrowthfactorthatexertsitsactivitybysignalingthroughthec-Kitreceptor.SCFandc-Kitareessentialforthesurvival,proliferationanddifferentiationofhematopoieticcellscommittedtothemelanocyteandgermcelllineages.HumanSCFmanifestslowactivityonmurinecells,whilemurineandratSCFarefullyactiveonhumancells.ThehumanSCFgeneencodesfora273 aminoacidtransmembraneprotein,whichcontainsa25 aminoacidN-terminalsignalsequence,a189 aminoacidextracellulardomain,a23 aminoacidtransmembranedomain,anda36 aminoacidcytoplasmicdomain.ThesecretedsolubleformofSCFisgeneratedbyproteolyticprocessingofthemembraneanchoredprecursor.RecombinantHumanSCFisan18.4 kDapolypeptidecontaining165 aminoacidresidues,whichcorrespondstothesequenceofthesecretedsolubleformof SCF.
AminoAcidSequence
MEGICRNRVTNNVKDVTKLVANLPKDYMITLKYVPGMDVLPSHCWISEMVVQLSDSLTDLLDKFSNISEGLSNYSIIDKLVNIVDDLVECVKENSSKDLKKSFKSPEPRLFTPEEFFRIFNRSIDAFKDFVVASETSDCVVSSTLSPEKDSRVSVTKPFMLPPVA
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50asdeterminedbythedose-dependentstimulationoftheproliferationofhumanTF-1 cellsis≤2.0 ng/ml,correspondingtoaspecificactivityof≥5×105 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
