Type

Recombinant

Description

CD30ligand(CD30L)isatype-IImembrane-associatedglycoproteinbelongingtotheTNFsuperfamilyandisexpressedprimarilyoncertainBcells,Tcells,andmonocytes.CD30LbindsspecificallytoCD30(receptor),whichisexpressedonactivated,butnotresting,BandTcells,inlymphomasandvariouschronicallyinflamedtissues.CD30L/CD30interactionsinitiateasignalingcascadethatcanultimatelyleadtotheactivationofNF-κB.CD30L/CD30signalingexertspleiotropiceffectsonnormalcells,includingcelldeath,differentiation,andcelldivision.Certaindiseases,includingHodgkin’s lymphoma,allergicinflammation,diabetes(inNODmice),andmycobacterialinfectioncanalsobeaffectedbyCD30L/CD30signaling.TheCD30Lgeneencodesfora234 aminoacidtypeIItransmembraneprotein,whichcontainsa37 aminoacidcytoplasmicsequence,a25 aminoacidtransmembranedomainanda172 aminoacidextracellulardomain.RecombinantHumansolubleCD30L(sCD30L)isa188 aminoacidpolypeptidecorrespondingtotheextracellulardomain,andcontainsan8 residueN-terminalHis-Tag.ThecalculatedmolecularweightofRecombinantHumansCD30Lis21.3 kDa.

AminoAcidSequence

HHHHHHHHPSPGGSGGQRTDSIPNSPDNVPLKGGNCSEDLLCILKRAPFKKSWAYLQVAKHLNKTKLSWNKDGILHGVRYQDGNLVIQFPGLYFIICQLQFLVQCPNNSVDLKLELLINKHIKKQALVTVCESGMQTKHVYQNLSQFLLDYLQVNTTISVNVDTFQYIDTSTFPLENVLSIFLYSNSD

Source

ChineseHamsterOvaryCells(CHO)

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytostimulatehumanIL-8 productionbyhumanPBMCusingaconcentrationrangeof10.0–25.0 ng/ml.Note:ResultsmayvarywithPBMCdonors.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C