Type
Recombinant
Description
CD23,thelowaffinityreceptorforIgE,belongstotheC-typelectinstructuralfamilyandplaysaroleintheregulationofIgEsynthesisandIgEmediatedactivities.Itisfoundbothasatransmembranereceptorproteinandinasolubleform,whichisgeneratedbyproteolyticcleavageofmembraneboundCD23.ThepredominantsolubleformofCD23(sCD23)consistsof172 aminoacidscorrespondingtotheextracellulardomainofthefulllengthprecursor.sCD23,inadditiontobindingIgE,alsoexertsanumberofIgE-independentactivities,suchaspromotingtheactivationanddifferentiationofB-cellsandstimulatingthereleaseofpro-inflammatorycytokinesfrommonocytes.RecombinantHumansCD23isa19.2 kDanon-glycosylatedproteincontaining172 amino-acidresidues.
AminoAcidSequence
MELQVSSGFVCNTCPEKWINFQRKCYYFGKGTKQWVHARYACDDMEGQLVSIHSPEEQDFLTKHASHTGSWIGLRNLDLKGEFIWVDGSHVDYSNWAPGEPTSRSQGEDCVMMRGSGRWNDAFCDRKLGAWVCDRLATCTPPASEGSAESMGPDSRPDPDGRLPTPSAPLHS
Source
E.coli
Purity
96%
BIOLOGicalActivity
MeasuredbyitsABIlitytoinduceTNF-αproductionbyhuman PBMCs.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C