Type

Recombinant

Description

CD14isacellsurface-anchoredglycoproteinthatisexpressedpredominantlybymonocytesandtissuemacrophages.CD14associateswithMD-2(LY-96)andTLR4toformareceptorcomplex,whichsignalsspecificallyinresponsetobacteriallipopolysaccharide(LPS)binding.TheCD14/MD-2/TLR4receptorcomplexsignalsviaMyD88,TIRAP,andTRAF6,andultimatelyactivatesNF-κB.CD14alsoexistsinasolubleform,designatedassCD14,whichiscapableofspecificallybindingLPSintheextracellularspace.RecombinantsCD14isa331 aminoacidglycoproteincomprisingtheextracellularportionoftheCD14receptor.ThecalculatedmolecularweightofRecombinantHumansCD14is35.6 kDa.

AminoAcidSequence

TTPEPCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRVDADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKELTLEDLKITGTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQWLKPGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERGLMAALCPHKFPAIQNLALRNTGMETPTGVCAALAAAGVQPHSLDLSHNSLRATVNPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKLRVLDLSCNRLNRAPQPDELPEVDNLTLDGNPFLVPGTALPHEGSMNSGVVP

Source

HEK293cells

Purity

95%

BIOLOGicalActivity

DeterminedbythedosedependentactivationofNF-κBinaRAW264celllinebasedreportersystem,usingasCD14concentrationrangeof20 ng/μlto200 ng/μl.TheNF-κBactivationisenhancedwhentheassayisdoneinthepresenceof0.25 ng/μlto1.0 ng/μlbacterial LPS.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C