Type

Recombinant

Description

TheSemphorinsarealargefamilyofphylogeneticallyconservedproteinsthatplayapivotalroleinmaintaininghomeostasisintheimmunesystem.Twentymembersofthisfamilyhavebeenidentifiedandcategorizedintoeightsubclassesbasedonsequencesimilarityanddistinctivestructuralfeatures.CD100,alsoknownasSema4D,isa150 kDatransmembraneclassIVsemaphorin.StudieshaveshownthatCD100caninducemonocytemigration,T-cellactivation,andB-cellsurvival,aswellasT/BcellandT/DC“cooperation”.TheCD100precursorcontains862 aminoacids,includinga21 a.a.signalsequence,a713 a.a.extracellulardomain,a21 a.a.transmembranesequence,anda107 a.a.cytoplasmicregion.Theextracellularsequencecontainsseveralstructuralfeatures,includinga479 a.a.“sema”domain,a79 a.a.Ig-likesequence,anda52 a.a.“Plexin-typerepeat”.RecombinantHumansolubleCD100isa78.9 kDaproteincomprisingtheextracellulardomainofCD100(711 aminoacids).SDS-PAGEanalysisrunundernon-reducingconditionsshowsamixtureofdisulfidelinkeddimerandmonomer.

AminoAcidSequence

FAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLHSEKTMYLKSSDNR

Source

ChineseHamsterOvaryCells(CHO)

Purity

97%

BIOLOGicalActivity

MeasuredbyitsABIlitytoinhibitchemokine(hMCP-3)inducedhumanmonocytemigration.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C