Type
Recombinant
Description
R-Spondin-1(Rspo-1)belongstothe(Rspo)familyofWntmodulators.Currently,thefamilyconsistsoffourstructurallyrelatedsecretedligands(Rspo1–4),allcontainingthefurin-likeandthrombospondinstructuraldomains.Rspo-1 isexpressedincertainareasofthedevelopingcentralnervoussystem,aswellasintheadrenalglands,ovary,testis,thyroid,andtrachea.RspocaninteractwiththeFrizzled/LRP6receptorcomplexinamannerthatstimulatestheWnt/beta-cateninsignalingpathway.RecombinantHumanR-Spondin-1 isa26.7 kDaproteinconsistingof243 aminoacidresidues.Duetoglycosylation,R-Spondin-1 migratesatanapparentmolecularweightofapproximately40.0 kDabySDS-PAGEanalysisunderreducingconditions.
AminoAcidSequence
SRGIKGKRQRRISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCKEGLYLHKGRCYPACPEGSSAANGTMECSSPAQCEMSEWSPWGPCSKKQQLCGFRRGSEERTRRVLHAPVGDHAACSDTKETRRCTVRRVPCPEGQKRRKGGQGRRENANRNLARKESKEAGAGSRRRKGQQQQQQQGTVGPLTSAGPA
Source
ChineseHamsterOvaryCells(CHO)
Purity
95%
BIOLOGicalActivity
R-spondin-1 enhancesBMP-2 mediateddifferentiationofMC3T3-E1cells.TheexpectedED50is1.0–3.0 ug/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
