Type

Recombinant

Description

Total 204 AA. MW: 23 kDa (calculated). N-terminal His-tag, 21 extra AA (highlighted). The AA sequence is identical to UniProtKB/Swiss-Prot entry Q5VY30 (AA16–199).

Amino Acid Sequence

MSWWHHHHHHNWNIPTTQDTTERDCRVSSFRVKENFDKARFSGTWYAMAKKDPEGLFLQDNIVAEFSVDETGQMSATAKGRVRLLNNWDVCADMVGTFTDTEDPAKFKMKYWGVASFLQKGNDDHWIVDTDYDTYAVQYSCRLLNLDGTCADSYSFVFSRDPNGLPPEAQKIVRQRQEELCLARQYRLIVHNGYCDGRSERNLL

Source

E. coli

Purity

> 95%, as determined by SDS-PAGE.

SDS-PAGE Gel

12% SDS-PAGE separation of Human RBP4
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 5μg / lane
3. non-reduced and non-heated sample, 5μg / lane

Biological Activity

Determined by its ability to bind all trans retinol acids. The binding of RBP4 to all trans retinol acids results in quenching of Trp fluroscence. 0.5 M retinol acids/per M RBP4.

Endotoxin

<0.5 EU/ug protein.

Formulation

Filtered (0.4 μm) and lyophilized from 0.5 mg/ml in PBS buffer, pH 7.5

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

Applications

Western blotting, ELISA, Binding assay

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Note

This product is intended for research use only.