Type

Recombinant

Description

Total110 AA.MW:11.9 kDa(calculated).N-TerminalHis-tag16 AA(highlighted).

AminoAcidSequence

MRGSHHHHHHGMASHMPSMSLCPMDEAISKKINQDFSSLLPAAMKNTVLHCWSVSSRGRLASCPEGTTVTSCSCGSGCGSWDVREDTMCHCQCGSIDWTAARCCTLRVGS

Source

E.coli

Purity

>95%

SDS-PAGEGel

12%SDS-PAGEseparationofRatResistin
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,10μg/lane
3.non-reducedandnon-heatedsample,10μg/lane

Formulation

Filtered(0,4 μm)andlyophilizedin0.5 mg/mLin20mMTrispH 8.0

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.

Applications

Westernblotting,ELISA

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

Note

Thisproductisintendedforresearchuseonly.