Type

Recombinant

Description

Total103 AA.MW:11.3 kDa(calculated).UniProtKBacc.No.Q99P87(Ser21–Ser114).N-terminalHis-tag(9 extraAA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

MKHHHHHHASSMPLCPIDEAIDKKIKQDFNSLFPNAIKNIGLNCWTVSSRGKLASCPEGTAVLSCSCGSACGSWDIREEKVCHCQCARIDWTAARCCKLQVAS

Source

E.coli

Purity

Purityasdeterminedbydensitometricimageanalysis: >95%

SDS-PAGEGel

14%SDS-PAGEseparationofMouseResistin
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,2.5μg/lane
3.non-reducedandnon-heatedsample,2.5μg/lane

Endotoxin

<0.1 EU/µg

Formulation

Filtered(0.4 μm)andlyophilizedin0.5 mg/mLin0.05 MAcetatebuffer pH=4.0

Reconstitution

Add0.1MAcetatebufferpH4toprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.ForconversionintohigherpHvalue,werecommendintensivedilutionbyrelevantbuffertoaconcentrationof10μg/mL.Inhigherconcentrationsthesolubilityofthisantigenislimited.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.Therecombinantproteinisforresearchuse only.