Type
Recombinant
Description
Relaxin-3 isasecretedproteinstructurallyrelatedtoinsulinthatisexpressedprimarilyinthebrainandcentralnervoussystem.Relaxin-3 hasbeenidentifiedastheligandfortheGPCR135receptor,previouslyknownas“somatostatin-like”or“angiotensin-like”peptidereceptor,andhasalsobeenidentifiedforbindingspecificallytotheLGR7receptor,previouslyidentifiedasan“orphan”Gprotein-coupledreceptor.Signalingbyrelaxin-3 throughitstargetreceptorsis,mostlikely,partofaCNSprocessingsystem,activatedinresponsetosignalingbyneuropeptidesandotherfactors.Intracerebroventricularinjectionsofrelaxin-3 havebeenshowntocauseasignificantincreaseoffoodintakeandbodyweightinWistarrats.RecombinantHumanRelaxin-3 isa5.5 kDa,disulfide-linked,heterodimericproteinconsistingofa24 aminoacidA-chainanda27 aminoacidB-chain.
Source
E.coli
Purity
98%
BIOLOGicalActivity
DataNotAvailable.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
