Type

Recombinant

Description

RANTESisaCCchemokinethatcansignalthroughtheCCR1,CCR3,CCR5andUS28(cytomegalovirusreceptor)receptors.Itisachemoattractanttowardsmonocytes,memoryTcells(CD4+/CD45RO),basophils,andeosinophils.RANTESalsohasthecapABIlitytoinhibitcertainstrainsofHIV-1,HIV-2 andsimianimmunodeficiencyvirus(SIV).RecombinantMurineRANTESisa7.8 kDaproteincontaining68 aminoacidresidues,includingthefourhighlyconservedcysteineresiduespresentintheCCchemokines.

AminoAcidSequence

SPYGSDTTPCCFAYLSLALPRAHVKEYFYTSSKCSNLAVVFVTRRNRQVCANPEKKWVQEYINYLEMS

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsabilitytochemoattracttotalhumanlymphocytepopulationandtotalmurineTcellpopulationusingaconcentrationrangeof1.0–10.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C