Type

Recombinant

Description

Prolactinisaneuroendocrinehormonesecretedbythepituitarygland.Itsprimaryfunctionistopromoteandmaintainlactationduringpregnancyandsuckling.Inaddition,prolactinplaysanimmunoegulatoryrolebystimulatingtheactivitiesofornithinedecarboxylaseandproteinkinaseC,whichareimportantfortheproliferation,differentiation,andfunctionoflymphocytes.RecombinantRatProlactinisa22.6 kDaglobularproteincontaining198 aminoacidresidues.

AminoAcidSequence

MLPVCSGGDCQTPLPELFDRVVMLSHYIHTLYTDMFIEFDKQYVQDREFIAKAINDCPTSSLATPEDKEQAQKVPPEVLLNLILSLVHSWNDPLFQLITGLGGIHEAPDAIISRAKEIEEQNKRLLEGIEKIISQAYPEAKGNEIYLVWSQLPSLQGVDEESKDLAFYNNIRCLRRDSHKVDNYLKFLRCQIVHKNNC

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytoinducetheproliferationofratNb2–11cellsintheconcentrationrangeof0.1–1.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C