Type

Recombinant

Description

ProlactinHumanRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining200 aminoacidsandhavingamolecularmassof23007 Dalton.TheProlactinispurifiedbyproprietarychromatographictechniques.

AminoAcidSequence

ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeMet-Leu-Pro-Ile-Cys

Source

E.coli

Purity

Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.

BIOLOGicalActivity

TheED50asdeterminedbythedose-dependantstimulationoftheproliferationofratlymphoma,Nb2–11wasfoundtobe<0.065 ng/ml.

Formulation

Theproteinwaslyophilizedfromaconcentrated(1 mg/ml)solutionwith10 mMsodiumPhosphatebufferpH=8and50 mM NaCl.

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

LyophilizedProlactinalthoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionProlactinshouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.

PhysicalAppearance

Sterilefilteredwhitelyophilized(freeze-dried)powder.