Type

Recombinant

Description

PLGF-2 isanangiogenicfactorthatbelongstothecysteine-knotsuperfamilyofgrowthfactors.PLGF-2 isexpressedinumbilicalveinendothelialcellsandplacenta.ItsignalsthroughtheVEGFR-1/FLT1receptor,andstimulatesendothelialcellproliferationandmigration.PlGF-2 alsosignalsthroughNeuropilin(NP-1),andcanbindwithhighaffinitytoheparin.RecombinantHumanPLGF-2 isa34.0 kDadisulfide-linkedhomodimericproteinoftwo150 aminoacidpolypeptidechains.

AminoAcidSequence

LPAVPPQQWALSAGNGSSEVEVVPFQEVWGRSYCRALERLVDVVSEYPSEVEHMFSPSCVSLLRCTGCCGDENLHCVPVETANVTMQLLKIRSGDRPSYVELTFSQHVRCECRPLREKMKPERRRPKGRGKRRREKQRPTDCCGDAVPRR

Source

E.coli

Purity

95%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanmonocytesofusingaconcentrationrangeof5.0–50.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C