Type

Recombinant

Description

PlGF-1 isanangiogenicfactorthatbelongstothecysteine-knotsuperfamilyofgrowthfactors.PlGF-1 isexpressedinplacentaltissues,thecolon,andmammarycarcinomas.ItsignalsthroughtheVEGFR-1/FLT1receptor,andstimulatesendothelialcellproliferationandmigration.RecombinantHumanPlGF-1 isa29.7 kDadisulfide-linkedhomodimericproteinoftwo132 aminoacidpolypeptidechains.

AminoAcidSequence

MLPAVPPQQWALSAGNGSSEVEVVPFQEVWGRSYCRALERLVDVVSEYPSEVEHMFSPSCVSLLRCTGCCGDENLHCVPVETANVTMQLLKIRSGDRPSYVELTFSQHVRCECRPLREKMKPERCGDAVPRR

Source

E.coli

Purity

95%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanmonocytesofusingaconcentrationrangeof1.0–10.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C