Type

Recombinant

Description

Total424 AA.MW:48.4 kDa(calculated).UniProtKBacc.No.P16671(Gly30–Asn439).N-terminallinker(2 extraAA),C-terminallinker(2 extraAA)andC-terminalHis-tag(10 extraAA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

ASGDLLIQKTIKKQVVLEEGTIAFKNWVKTGTEVYRQFWIFDVQNPQEVMMNSSNIQVKQRGPYTYRVRFLAKENVTQDAEDNTVSFLQPNGAIFEPSLSVGTEADNFTVLNLAVAAASHIYQNQFVQMILNSLINKSKSSMFQVRTLRELLWGYRDPFLSLVPYPVTTTVGLFYPYNNTADGVYKVFNGKDNISKVAIIDTYKGKRNLSYWESHCDMINGTDAASFPPFVEKSQVLQFFSSDICRSIYAVFESDVNLKGIPVYRFVLPSKAFASPVENPDNYCFCTEKIISKNCTSYGVLDISKCKEGRPVYISLPHFLYASPDVSEPIDGLNPNEEEHRTYLDIEPITGFTLQFAKRLQVNLLVKPSEKIQVLKNLKRNYIVPILWLNETGTIGDEKANMFRSQVTGKINKLHHHHHHHHHH

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis: >90%

SDS-PAGEGel

12 %SDS-PAGEseparationofHumanPlateletGlycoprotein4:
1. MWMarker –14,21,31,45,66,97 kDa
2.Reducedandboiledsample,2.5 μg/lane
3.Non-reducedandnon-boiledsample,2.5 μg/lane

Endotoxin

<1.0 EU/ug

Formulation

0.3 –0.5 mg/mlinPBS,pH=7.4,5 %trehalose –filtered(0.4 μm), frozen

Reconstitution

Defrostatambienttemperature.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA

Shipping

Onice.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storeproteinat –80°C.Proteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Avoidrepeatedfreezing/thawingcycles.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.