Type

Recombinant

Description

Total410 AA.Mw:45.6 kDa(calculated).C-terminalFlag-tag11AA(highlighted).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

QNPASPPEEGSPDPDSTGALVEEEDPFFKVPVNKLAAAVSNFGYDLYRVRSSTSPTTNVLLSPLSVATALSALSLGAEQRTESIIHRALYYDLISSPDIHGTYKELLDTVTAPQKNLKSASRIVFEKKLRIKSSFVAPLEKSYGTRPRVLTGNPRLDLQEINNWVQAQMKGKLARSTKEIPDEISILLLGVAHFKGQWVTKFDSRKTSLEDFYLDEERTVRVPMMSDPKAVLRYGLDSDLSCKIAQLPLTGSMSIIFFLPLKVTQNLTLIEESLTSEFIHDIDRELKTVQAVLTVPKLKLSYEGEVTKSLQEMKLQSLFDSPDFSKITGKPIKLTQVEHRAGFEWNEDGAGTTPSPGLQPAHLTFPLDYHLNQPFIFVLRDTDTGALLFIGKILDPRGPAAADYKDDDDK

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis: >95%

SDS-PAGEGel

12%SDS-PAGEseparationofHumanPEDF
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,7μg/lane
3.non-reducedandnon-heatedsample,7μg/lane

Endotoxin

<0.1 EU/µg

Formulation

Filtered(0.4 μm)andlyophilizedin0.5 mg/mLin20mMTRIS,50mMNaCl,pH 7.5

Reconstitution

Add200 µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.