Type
Recombinant
Description
Theplatelet-derivedgrowthfactor(PDGF)familyofheparin-bindinggrowthfactorsconsistsoffiveknownmembers,denotedPDGF-AA,PDGF-BB,PDGF-AB,PDGF-CCandPDGF-DD.Thematureandactiveformoftheseproteins,ananti-parallel,disulfide-linkeddimeroftwo12–14kDa,polypeptidechains,isobtainedthroughproteolyticprocessingofBIOLOGicallyinactiveprecursorproteins,whichcontainanN-terminalCUBdomainandaPDGF/VEGFhomologousdomain.ThePDGFsinteractwithtworelatedproteintyrosinekinasereceptors,PDGFR-αandPDGFR-β,andarepotentmitogensforavarietyofcelltypes,includingsmoothmusclecells,connectivetissuecells,boneandcartilagecells,andcertaintumorcells.Theyplayanimportantroleinanumberofbiologicalprocesses,includinghyperplasia,chemotaxis,embryonicneurondevelopment,andrespiratorytubules"epithelialcelldevelopment.MaturePDGFsarestoredinplateletα-granules,andarereleaseduponplateletactivation.PDGF-AA,-AB,-BBand –CCsignalprimarilythroughthePDGF-Rαreceptor,whereasPDGF-DDinteractsalmostexclusivelywiththePDGF-Rβreceptor.RecombinantHumanPDGF-CCisa25.0 kDaproteinconsistingoftwoidenticaldisulfide-linked,112 aminoacid,polypeptidechains.
AminoAcidSequence
MVVDLNLLTEEVRLYSCTPRNFSVSIREELKRTDTIFWPGCLLVKRCGGNCACCLHNCNECQCVPSKVTKKYHEVLQLRPKTGVRGLHKSLTDVALEHHEECDCVCRGSTGG
Source
E.coli
Purity
98%
BiologicalActivity
Determinedbythedose-dependentstimulationoftheproliferationofBalb/c3T3cells. TheexpectedTheED50forthiseffectis15–20 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C