Type
Recombinant
Description
PDGFsaredisulfide-linkeddimersconsistingoftwo12.0–13.5 kDapolypeptidechains,designatedPDGF-A andPDGF-Bchains.ThethreenaturallyoccurringPDGFs,PDGF-AA,PDGF-BBandPDGF-AB,arepotentmitogensforavarietyofcelltypes,includingsmoothmusclecells,connectivetissuecells,boneandcartilagecells,andsomebloodcells.ThePDGFsarestoredinplateletα-granules,andarereleaseduponplateletactivation.ThePDGFsareinvolvedinanumberofBIOLOGicalprocesses,includinghyperplasia,chemotaxis,embryonicneurondevelopment,andrespiratorytubuleepithelialcelldevelopment.TwodistinctsignalingreceptorsusedbyPDGFshavebeenidentifiedandnamedPDGFR-αandPDGFR-β.PDGFR-αisahigh-affinityreceptorforeachofthethreePDGFforms.Ontheotherhand,PDGFR-βinteractswithonlyPDGF-BBandPDGF-AB.RecombinantHumanPDGF-AAisa28.5 kDadisulfide-linkedhomodimeroftwoA chains(250 totalaminoacids).
AminoAcidSequence
SIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATTSLNPDYREEDTGRPRESGKKRKRKRLKPT
Source
E.coli
Purity
98%
BiologicalActivity
TheED50asdeterminedbythedose-dependentstimulationofthymidineuptakebyBalb/c3T3cellsis1 ng/ml,correspondingtoaspecificactivityof1×106units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
