Type

Recombinant

Description

PAI-2 isaninhibitoryserpinexpressedmainlyinkeratinocytes,activatedmonocytes,andplacentaltrophoblasts.Itexistspredominantlyasa47 kDa,nonglycosylated,intracellularprotein,whichcanbeinducedtobesecretedas60 kDaglycoprotein.TheglycosylatedandunglycosylatedformsofPAI-2 areequallyeffectiveasinhibitorsofurokinase-typeplasminogenactivator(uPA),theonlyestablishedphysiologicaltargetofthisserpin.PAI-2 hasauniqueABIlitytoformdormantpolymersspontaneouslyandreversIBLyunderphysiologicalconditions.Thephysiologicalrelevanceofthisproperty,whichisneitheraconsequenceofanymutationinthePAI-2 genenorassociatedwithanyknowndisorder,isstillunclear.However,itappearsthattheformationofintracellular,dormantpolymersmaybeimportantforthecontrolledreleaseoftheinhibitorfromPAI-2 producingcells.PlasmalevelsofPAI-2 areusuallyloworundetectable,exceptduringpregnancyandinsomeformsofmonocyticleukemia.SecretionofPAI-2 fromtheplacentanormallyoccursduringthethirdtrimesterofpregnancy,andaccountsforthedramaticincreaseinPAI-2 levels(upto250 ng/ml),whicharemaintainedattheselevelsuntilpostpartum,andthenrapidlydecline.InadditiontoitsvitalroleinprotectingtheplacentafromdegradationbyuPAand/oruPA-activatedproteases,PAI-2 hasbeenshowntobeessentialforthepreventionofmetastaticspreadofneck,lungandbreastcancers.ThebeneficialeffectofPAI-2 seeninthesestudiesispresumedtostemfromitsabilitytoinhibituPA-dependentcelldissemination.PAI-2 hasalsobeenreportedtoinhibitkeratinocyteproliferation,andtoparticipateintheinnateimmuneresponseduringviralinfection.RecombinantHumanPAI-2 isa46.5 kDa,nonglycosylatedproteinof415 residues.

AminoAcidSequence

MEDLCVANTLFALNLFKHLAKASPTQNLFLSPWSISSTMAMVYMGSRGSTEDQMAKVLQFNEVGANAVTPMTPENFTSCGFMQQIQKGSYPDAILQAQAADKIHSSFRSLSSAINASTGNYLLESVNKLFGEKSASFREEYIRLCQKYYSSEPQAVDFLECAEEARKKINSWVKTQTKGKIPNLLPEGSVDGDTRMVLVNAVYFKGKWKTPFEKKLNGLYPFRVNSAQRTPVQMMYLREKLNIGYIEDLKAQILELPYAGDVSMFLLLPDEIADVSTGLELLESEITYDKLNKWTSKDKMAEDEVEVYIPQFKLEEHYELRSILRSMGMEDAFNKGRANFSGMSERNDLFLSEVFHQAMVDVNEEGTEAAAGTGGVMTGRTGHGGPQFVADHPFLFLIMHKITNCILFFGRFSSP

Source

E.coli

Purity

95%

BIOLOGicalActivity

DeterminedbyitsinhibitoryeffectagainstsinglechaintPAinducedcleavageofachromogenicsubstrateinImidazoleBufferat37°C.Halfmaximalinhibitionagainst1.0 µg/mlofsinglechaintPAwasobtainedataconcentrationof1.0 µg/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.

Storage/Expiration

 –20°C