Type

Recombinant

Description

p16-INK4aisanuclearproteinthatregulatesthecellcyclebyinhibitingcyclin-dependentkinase-4(CDK4)andCDK6.p16-INK4ainhibitsCDKactivitybybindingtotheCDKmoleculesinamannerthatinterfereswiththeirABIlitytointeractwithcyclinD.Thisactivityhastheeffectofsuppressingtumorformationandgrowth,andofinducingreplicativesenescenceinvariousnormalcells,includingstemcells.Theexpressionofp16-INK4asteADIlyincreaseswithage,andtendstoaccumulateinstemcellcompartments.Thedeletion,rearrangement,ormutationofthep16-INK4ageneisfrequentlyfoundinmelanomas,aswellasincertainothertypesofcancer.p16-INK4aandothertranscriptionfactorshavebeenintroducedintocellsbyDNAtransfection,viralinfection,ormicroinjection.ProteintransductionusingTATfusionproteinsrepresentsanalternativemethodologyforintroducingtranscriptionfactorsandothernuclearproteinsintoprimary,aswellastransformed,cells.RecombinantHumanp16-INK4a-TATexpressedinE.coliisan18.0 kDaproteincontaining167 amino-acidresidues,includingthe155 residuesoffull-lengthp16-INK4aanda12-residueC-terminalTATpeptide(GYGRKKRRQRRR).

AminoAcidSequence

EPAAGSSMEPSADWLATAAARGRVEEVRALLEAGALPNAPNSYGRRPIQVMMMGSARVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEELGHRDVARYLRAAAGGTRGSNHARIDAAEGPSDIPDGYGRKKRRQRRR

Source

E.coli

Purity

95%

BIOLOGicalActivity

Datanotavailable.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C