Type

Recombinant

Description

p16-INK4aisanuclearproteinthatregulatesthecellcyclebyinhibitingcyclin-dependentkinase-4(CDK4)andCDK6.p16-INK4ainhibitsCDKactivitybybindingtotheCDKmoleculesinamannerthatinterfereswiththeirABIlitytointeractwithcyclinD.Thisactivityhastheeffectofsuppressingtumorformationandgrowth,andofinducingreplicativesenescenceinvariousnormalcells,includingstemcells.Theexpressionofp16-INK4asteADIlyincreaseswithage,andtendstoaccumulateinstemcellcompartments.Thedeletion,rearrangement,ormutationofthep16-INK4ageneisfrequentlyfoundinmelanomas,aswellasincertainothertypesofcancer.RecombinantHumanp16-INK4aisa16.5 kDaproteincontaining156 aminoacidresidues.Kim,W.Y.andSharpless,N.E.(2006)Cell127(2):265–75.

AminoAcidSequence

MEPAAGSSMEPSADWLATAAARGRVEEVRALLEAGALPNAPNSYGRRPIQVMMMGSARVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEELGHRDVARYLRAAAGGTRGSNHARIDAAEGPSDIPD

Source

E.coli

Purity

95%

BIOLOGicalActivity

Datanotavailable.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstitutein1×PBS,pH8.0 toaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C