Type

Recombinant

Description

Total221 AA.MW:25.3 kDa(calculated).UniProtKBacc.No.P78380(Ser61–Gln273).N-terminalflag-tag(8 AA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

DYKDDDDKSQVSDLLTQEQANLTHQKKKLEGQISARQQAEEASQESENELKEMIETLARKLNEKSKEQMELHHQNLNLQETLKRVANCSAPCPQDWIWHGENCYLFSSGSFNWEKSQEKCLSLDAKLLKINSTADLDFIQQAISYSSFPFWMGLSRRNPSYPWLWEDGSPLMPHLFRVRGAVSQTYPSGTCAYIQRGAVYAENCILAAFSICQKKANLRAQ

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis:>95 %

SDS-PAGEGel

12 %SDS-PAGEseparationofHumansLOX1(Flagtag):
1. M.W.Marker –14,21,31,45,66,97 kDa
2.Reducedandboiledsample,2.5 μg/lane
3.Non-reducedandnon-boiledsample,2.5 μg/lane

Endotoxin

<1.0 EU/μg

Formulation

Filtered(0.4 μm)andlyophilizedfromphosphatebufferedsalinepH 7.5.

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.