Type
Recombinant
Description
RecombinantOPGproducedinyeastcontains412 aminoacidresidues,including180 residuesfrommatureOPG(a.a22–201)and232 residuesfromtheFcproteinofhumanIgG1,andhasacalculatedmolecularmassof46.5 kDa.Asaresultofglycosylation,therecombinantOsteoprotegrinmigratesasa49 kDaproteininSDS-PAGEunderreducingconditions.TheOPGispurifiedbyproprietarychromatographictechniques.
AminoAcidSequence
OPG22–201:ETFPPKYLHYDEETSHQLLCDKCPPGTYLKQHCTAKWKTVCAPCPDHYYTDSWHTSDECLYCSPVCKELQYVKQECNRTHNRVCECKEGRYLEIEFCLKHRSCPPGFGVVQAGTPERNTVCKRCPDGFFSNETSSKAPCRKHTNCSVFGLLLTQKGNATHDNICSGNSESTQKCGIDVTL.Fc232:EPKSSDKTHTCPPCPAPEFEGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPTPIEKTISKAKGQREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
Source
PichiaPastoris
Purity
Greaterthan90.0%asdeterminedby(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
BIOLOGicalActivity
DeterminedbyitsABIlitytoneutralizethestimulationofU937cellstreatedwith10ng/mlofsolubleRANKL(sRANKL).
Formulation
OPGwaslyophilizedfroma0.2?mfilteredconcentrated(0.5mg/ml)solutioninPBS,pH= 7.4.
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
ItisrecommendedtoreconstitutethelyophilizedOsteoprotegerininsterile18 M?-cmH2Onotlessthan100 µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
PhysicalAppearance
Sterilefilteredwhitelyophilized(freeze-dried)powder.
