Type
Recombinant
Description
Total393AA.MW:45.0kDa(calculated).UniProtKBacc.No.O00300(Glu22–Leu401).N-terminalFLAG-tag(13extraAA).ProteinidentityconfirmedbyLC-MS/MS.
AminoAcidSequence
PGDYKDDDDKPAGETFPPKYLHYDEETSHQLLCDKCPPGTYLKQHCTAKWKTVCAPCPDHYYTDSWHTSDECLYCSPVCKELQYVKQECNRTHNRVCECKEGRYLEIEFCLKHRSCPPGFGVVQAGTPERNTVCKRCPDGFFSNETSSKAPCRKHTNCSVFGLLLTQKGNATHDNICSGNSESTQKCGIDVTLCEEAFFRFAVPTKFTPNWLSVLVDNLPGTKVNAESVERIKRQHSSQEQTFQLLKLWKHQNKDQDIVKKIIQDIDLCENSVQRHIGHANLTFEQLRSLMESLPGKKVGAEDIEKTIKACKPSDQILKLLSLWRIKNGDQDTLKGLMHALKHSKTYHFPKTVTQSLKKTIRFLHSFTMYKLYQKLFLEMIGNQVQSVKISCL
Source
HEK293
Purity
Purityasdeterminedbydensitometricimageanalysis:>90%
SDS-PAGEGel
12%SDS-PAGEseparationofHumanOPG:
- M.W.Marker–14,21,31,45,66,97kDa
- Reducedandboiledsample,2.5μg/lane
- Non-reducedandnon-boiledsample,2.5μg/lane
Endotoxin
<1.0 EU/µg
Formulation
Filtered(0.4μm)andlyophilizedinphosphatebufferedsalinepH7.5containing5%(v/v)Trehalose.
Reconstitution
Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.
Applications
Westernblotting,ELISA
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4°Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Thisproductisintendedforresearchuseonly.
