Biovendor/Osteoblast Specific Factor 2 Human E. coli/RD172045025/0.025 mg-免疫在线蚂蚁淘旗下平台

商品详细Biovendor/Osteoblast Specific Factor 2 Human E. coli/RD172045025/0.025 mg

Biovendor/Osteoblast Specific Factor 2 Human E. coli/RD172045025/0.025 mg

商品编号： RD172045025

品牌： Biovendor-Laboratorni

市场价： ¥2980.00

美元价： 1788.00

产地：美国(厂家直采)

公司：

产品分类：其它重组蛋白

公司分类： Other_recombinant_protein

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Regulatorystatus:RUO
Type:Recombinant
Source:E.coli
Othernames:Periostin,PN,OSF-2,POSTN,Fasciclin-Ilike,OsteoblastSpecificFactor1(Pleiotrophin,PTN,Heparin-bindinggrowth-associatedmolecule,HB-GAM,Heparin-bindinggrowthfactor8,HBGF-8,OSF-1,Heparin-bindingneuriteoutgrowth-promotingfactor1,HBNF-1,Heparin-bindingbrainmitogen,HBBM),NCK
Species:Human

Type

Recombinant

Description

Total671 AA.MW:75 kDa(calculated).UniProtKBacc.no.Q15063.N-TerminalHisTagandXa –cleavagesite23 AA(highlighted).

AminoAcidSequence

MGHHHHHHHHHHSSGHIEGRHMRNNHYDKILAHSRIRGRDQGPNVCALQQILGTKKKYFSTCKNWYKKSICGQKTTVLYECCPGYMRMEGMKGCPAVLPIDHVYGTLGIVGATTTQRYSDASKLREEIEGKGSFTYFAPSNEAWDNLDSDIRRGLESNVNVELLNALHSHMINKRMLTKDLKNGMIIPSMYNNLGLFINHYPNGVVTVNCARIIHGNQIATNGVVHVIDRVLTQIGTSIQDFIEAEDDLSSFRAAAITSDILEALGRDGHFTLFAPTNEAFEKLPRGVLERFMGDKVASEALMKYHILNTLQCSESIMGGAVFETLEGNTIEIGCDGDSITVNGIKMVNKKDIVTNNGVIHLIDQVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASALRPDGEYTLLAPVNNAFSDDTLSMVQRLLKLILQNHILKVKVGLNELYNGQILETIGGKQLRVFVYRTAVCIENSCMEKGSKQGRNGAIHIFREIIKPAEKSLHEKLKQDKRFSTFLSLLEAADLKELLTQPGDWTLFVPTNDAFKGMTSEEKEILIRDKNALQNIILYHLTPGVFIGKGFEPGVTNILKTTQGSKIFLKEVNDTLLVNELKSKESDIMTTNGVIHVVDKLLYPADTPVGNDQLLEILNKLIKYIQIKFVRGSTFKEIPVTVY

Source

E.coli

Purity

>90%

SDS-PAGEGel

12%SDS-PAGEseparationofHumanOSF-2
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,5μg/lane
3.non-reducedandnon-heatedsample,5μg/lane

Endotoxin

<1.0 EU/ug

Formulation

Filtered(0,4 μm)andlyophilizedin0.5 mg/mLin0.05 MAcetatebuffer pH=4.0

Reconstitution

Add0.1MAcetatebufferpH=4.0 toprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletelyat37°C.ForconversionintohigherpHvalue,werecommendintensivedilutionbyrelevantbuffertoaconcentrationof10μg/mL.Inhigherconcentrationsthesolubilityofthisantigenislimited.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA

Shipping

Onice.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

品牌介绍

miREIA是一种基于免疫测定的新型miRNA定量方法，涉及从患者样品中分离出的miRNA与互补的生物素化DNA寡核苷酸探针杂交。然后通过固定有完美匹配的DNA / miRNA杂交体的微量滴定板固定的单克隆抗体捕获DNA / RNA杂交体。下一步是复制标准ELISA方案。该测定可在常规免疫测定设备上运行，与标准临床工作流程兼容，不需要扩增步骤，并且包括miRNA分析在内的结果可在不到三个小时的时间内获得。