Type

Recombinant

Description

Total 152 AA. MW: 17.3 kDa. UniProtKB acc.no. P21246. N-Terminal His-tag and thrombin cleavage site 16 AA (highlighted).

Amino Acid Sequence

MKHHHHHHHMLVPRGSGKKEKPEKKVKKSDCGEWQWSVCVPTSGDCGLGTREGTRTGAECKQTMKTQRCKIPCNWKKQFGAECKYQFQAWGECDLNTALKTRTGSLKRALHNAECQKTVTISKPCGKLTKPKPQAESKKKKKEGKKQEKMLD

Source

E. coli

Purity

>95%

SDS-PAGE Gel

12% SDS-PAGE separation of Human OSF-1
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 10μg/lane
3. non-reduced and non-heated sample, 10μg/lane

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.1 M phosphate buffer, 0.1 M NaCl, pH 7.2

Reconstitution

Add PBS pH 7.2 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

Applications

Western blotting, ELISA

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Note

This product is intended for research use only.