Type
Recombinant
Description
OncostatinM(OSM)isagrowthanddifferentiationfactorthatparticipatesintheregulationofneurogenesis,osteogenesisandhematopoiesis.ProducedbyactivatedTcells,monocytesandKaposi’s sarcomacells,OSMcanexertbothstimulatoryandinhibitoryeffectsoncellproliferation.Itstimulatestheproliferationoffibroblasts,smoothmusclecellsandKaposi’s sarcomacells,butinhibitsthegrowthofsomenormalandtumorcelllines.Italsopromotescytokinerelease(e.g.IL-6,GM-CSFandG-CSF)fromendothelialcells,andenhancestheexpressionoflow-densitylipoproteinreceptorsinhepatomacells.OSMsharesseveralstructuralandfunctionalcharacteristicswithLIF,IL-6,andCNTF.RecombinantRatOncostatinMisa24.4 kDaprotein,containing215 aminoacidresidues.
AminoAcidSequence
MKRGCSSSSPKLLSQLKSQANITGNTASLLEPYILHQNLNTLTLRAACTEHPVAFPSEDMLRQLSKPDFLSTVHATLGRVWHQLGAFRQQFPKIQDFPELERARQNIQGIRNNVYCMARLLHPPLEIPEPTQADSGTSRPTTTAPGIFQIKIDSCRFLWGYHRFMGSVGRVFEEWGDGSRRSRRHSPLWAWLKGDHRIRPSRSSQSAMLRSLVPR
Source
E.coli
Purity
98%
BIOLOGicalActivity
Assay#1:DeterminedbyitsABIlitytostimulatetheproliferationofratC6cells. TheexpectedED50is3.0–5.0 µg/ml.Assay#2:DeterminedbyitsabilitytoinhibitAlkalinephosphataseactivityofdifferentiatedMC3T3E1 cells.
Storage/Expiration
–20°C
