Type
Recombinant
Description
OncostatinM(OSM)isagrowthanddifferentiationfactorthatparticipatesintheregulationofneurogenesis,osteogenesisandhematopoiesis.ProducedbyactivatedTcells,monocytesandKaposi’s sarcomacells,OSMcanexertbothstimulatoryandinhibitoryeffectsoncellproliferation.Itstimulatestheproliferationoffibroblasts,smoothmusclecellsandKaposi’s sarcomacells,butinhibitsthegrowthofsomenormalandtumorcelllines.Italsopromotescytokinerelease(e.g.IL-6,GM-CSFandG-CSF)fromendothelialcells,andenhancestheexpressionoflow-densitylipoproteinreceptorsinhepatomacells.OSMsharesseveralstructuralandfunctionalcharacteristicswithLIF,IL-6,andCNTF.HumanOSMisactiveonmurinecells.RecombinantHumanOncostatinMisa25.7 kDaprotein,containing227 aminoacidresidues(fulllengthprecursor).
AminoAcidSequence
AAIGSCSKEYRVLLGQLQKQTDLMQDTSRLLDPYIRIQGLDVPKLREHCRERPGAFPSEETLRGLGRRGFLQTLNATLGCVLHRLADLEQRLPKAQDLERSGLNIEDLEKLQMARPNILGLRNNIYCMAQLLDNSDTAEPTKAGRGASQPPTPTPASDAFQRKLEGCRFLHGYHRFMHSVGRVFSKWGESPNRSRRHSPHQALRKGVRRTRPSRKGKRLMTRGQLPR
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50asdeterminedbythedose-dependentstimulationoftheproliferationofhumanTF-1 cellsis2 ng/ml,correspondingtoaspecificactivityof5×105units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
