Type

Recombinant

Description

NT-4 isaneurotrophicfactorstructurallyrelatedtoβ-NGF,BDNF,andNT-3. Theseproteinsbelongtothecysteine-knotfamilyofgrowthfactorsthatassumestabledimericstructures.NT-4 isexpressedintheprostate,thymus,placenta,andskeletalmuscle.NT-4 cansignalthroughtheLNGFRandtrkBreceptors,andpromotesthesurvivalofperipheralsensorysympatheticneurons.RecombinantHumanNT-4 isanoncovalentlylinkedhomodimeroftwo14.0 kDapolypeptidemonomers(260 totalaminoacidresidues).

AminoAcidSequence

GVSETAPASRRGELAVCDAVSGWVTDRRTAVDLRGREVEVLGEVPAAGGSPLRQYFFETRCKADNAEEGGPGAGGGGCRGVDRRHWVSECKAKQSYVRALTADAQGRVGWRWIRIDTACVCTLLSRTGRA

Source

E.coli

Purity

98%

BIOLOGicalActivity

Determinedbythedose-dependentinductionofcholineacetyltransferaseactivityinratbasalforebrainprimaryseptalcellcultureswasfoundtobeintherangeof20–50 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C