Type

Recombinant

Description

Total253 AA.MW:29.1 kDa(calculated).UniProtKBacc.No.O14793(Asn24–Arg266).C-terminalHis-tag(10 extraAA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

NENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLRLETAPNISKDVIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIITMPTESDFLMQVDGKPKCCFFKFSSKIQYNKVVKAQLWIYLRPVETPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSRRHHHHHHHHHH

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis:> 80%

SDS-PAGEGel

14 %SDS-PAGEseparationofHumanMyostatinPropeptide:
1. M.W.Marker –14,21,31,45,66,97 kDa
2.Reducedandboiledsample,2.5 μg/lane
3.Non-reducedandnon-boiledsample,2.5 μg/lane

Endotoxin

<1.0 EU/ug

Formulation

Frosenfrom0,25–0,5mg/mLsolutioninphosphatebufferedsaline(PBS)pH8.0+20%(w/v)glycerol

Reconstitution

Defrostatambienttemperature.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies

Shipping

Onice.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storeproteinat –80°C.Proteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Avoidrepeatedfreezing/thawingcycles.Thethawedproteincanbestoredat4 °Cforthree days.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.