Type

Recombinant

Description

ArginineDeiminase(ADI)isamicrobialenzymefromMycoplasmaproducedinE.coli.IthashighaffinitytoL-arginineandhydrolyzesL-argininetocitrullineandammonia.LowconcentrationsofADIhavebeenshowntoinhibitproliferationincertainculturedcellsbyarrestingthecellcycleinG1and/orS phase.HigherconcentrationsofADIleadtosubsequentapoptosis.RecombinantMycoplasmaArginineDeiminaseisa46.3 kDaproteinconsistingof409 amino acids.

AminoAcidSequence

SVFDSKFKGIHVYSEIGELESVLVHEPGREIDYITPARLDELLFSAILESHDARKEHKQFVAELKANDINVVELIDLVAETYDLASQEAKDKLIEEFLEDSEPVLSEEHKVVVRNFLKAKKTSRELVEIMMAGITKTDLGIEADHELIVDPMPNLYFTRDPFASVGNGVTIHYMRYKVRQRETLFSRFVFSNHPKLINTPWYYDPSLKLSIEGGDVFIYNNDTLVVGVSERTDLQTVTLLAKNIVANKECEFKRIVAINVPKWTNLMHLDTWLTMLDKDKFLYSPIANDVFKFWDYDLVNGGAEPQPVENGLPLEGLLQSIINKKPVLIPIAGEGASQMEIERETHFDGTNYLAIRPGVVIGYSRNEKTNAALEAAGIKVLPFHGNQLSLGMGNARCMSMPLSRKDVKW

Source

E.coli

Purity

97%

BIOLOGicalActivity

MeasuredbyitsABIlitytoinduceapoptosisinJurkatcellsusingaconcentrationof100–150 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.

Storage/Expiration

 –20°C