Type
Recombinant
Description
MonocyteChemotacticProtein-1HumanRecombinantalsoknownasMonocyteChemotacticandActivatingFactor(MCAF)producedinE.coliisanon-glycosylated,Polypeptidechaincontaining76 aminoacidsandhavingamolecularmassof8607 Dalton.TheMCP-1 ispurifiedbyproprietarychromatographictechniques.
AminoAcidSequence
ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeGln-Pro-Asp-Ala-Ile.
Source
E.coli
Purity
Greaterthan98.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE
BIOLOGicalActivity
ThespecificactivityasdeterminedbytheABIlityofMCP-1 tochemoattracthumanMonocytesusingaconcentrationof5–20 ng/ml.
Formulation
Theproteinwaslyophilizedfromaconcentrated(1mg/ml)sterilesolutioncontainingnoadditives.
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
LyophilizedMCP-1 althoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionCCL2shouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
PhysicalAppearance
Sterilefilteredwhitelyophilized(freeze-dried)powder.
